Publication

Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

H.L. Klein, G. Bacinskaja, J. Che, A.Cheblal, R.Elango, A. Epshtein, D.M. Fitzgerald, B. Gomez-Gonzalez, S.R. Khan, S. Kumar, B.A. Leland, L. Marie, Q. Mei, J. Miné-Hattab, A.Piotrowska, E.J. Polleys, C.D. Putnam, E.A. Radchenko, A.A. Saada, C.J. Sakofsky, E.Y.Shim, M.Stracy,J. Xia, Z.Yan, Y. Yin, A. Aguilera, J.L. Argueso, C.H. Freudenreich, S. M. Gasser,4 Dmitry A. Gordenin, J. E. Haber, G. Ira, Sue Jinks-Robertson, M.C. King, R.D. Kolodner, A. Kuzminov, S.A Lambert, S. Eun Lee, K.M. Miller, S.M. Mirkin, T.D. Petes, S.M. Rosenberg, R. Rothstein, L.S. Symington, P. Zawadzki, N. Kim, M. Lisby, and A. Malkova

Published
January 7, 2019
journal
DOI
http://10.15698/mic2019.01.664
DNA repair
recombination
DNA damage
DNA breaks

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis.

These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

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Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
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