Publication

Variant-specific discrepancy when quantitating BCR-ABL1 e13a2 and e14a2 transcripts using the Europe Against Cancer qPCR assay

L. Kjaer, V. Skov, M.T. Andersen,A. Aggerholm, P.Clair, M. Gniot, K. Soeby, L. Udby, M.H. Dorff, H. Hasselbalch, N. Pallisgaard

Published
April 15, 2019
journal
DOI
https://doi.org/10.1111/ejh.13238
blood cancer
leukemia
BCR-ABL1
Diagnostics

Molecular monitoring of treatment response in patients with chronic myelogenous leukemia is performed using the Europe Against Cancer (EAC) qPCR assay using the International Scale (IS).

The assay amplifies both e13a2 and e14a2 BCR-ABL1 transcript variants. Observing distinct variant-dependent amplification curves during qPCR, we aimed to determine if this affected quantitation of BCR-ABL1.

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Variant-specific discrepancy when quantitating BCR-ABL1 e13a2 and e14a2 transcripts using the Europe Against Cancer qPCR assay
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